5F0Q

Crystal structure of C-terminal domain of the human DNA primase large subunit with bound DNA template/RNA primer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	C 2 2 21
Unit cell lengths	122.507, 126.002, 83.942
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.480 - 2.210
Rwork	0.223
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rr2
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.062	0.040	0.379
Rmeas	0.068	0.044	0.449
Rpim	0.027	0.017	0.235
Total number of observations	165370
Number of reflections	31361
<I/σ(I)>	9.6		1.55
Completeness [%]	95.4	99.4	58.3
Redundancy	5.3	6.2	2.7
CC(1/2)		0.995	0.745

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.18 M lithium sulfate, 50 mM sodium citrate, 2 mM TCEP, 0.1 M sodium malonate, 0.1 M Hepes-NaOH (pH 7.0), 19.7% PEG 8,000 and 0.02 M magnesium chloride