5F0P

Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1(L557M) (SeMet labeled)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97895
Spacegroup name	C 1 2 1
Unit cell lengths	371.290, 75.320, 57.270
Unit cell angles	90.00, 97.66, 90.00

Refinement procedure

Resolution	56.760 - 2.780
R-factor	0.2209
Rwork	0.219
R-free	0.26050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f0l
RMSD bond length	0.007
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	56.761	56.761	2.940
High resolution limit [Å]	2.770	8.230	2.770
Rmerge	0.066	0.024	0.904
Rmeas	0.078	0.029	1.073
Total number of observations	271917
Number of reflections	77507	2941	12013
<I/σ(I)>	12.17	44.87	1.33
Completeness [%]	99.1	99.1	95.3
CC(1/2)	0.999	0.999	0.808

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.8 M AmSO4, 0.1 M MES pH 6.0, 10% Glycerol