5F0E
Murine endoplasmic reticulum alpha-glucosidase II
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-20 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.96597 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 104.144, 173.773, 62.836 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.090 - 1.740 |
R-factor | 0.143 |
Rwork | 0.141 |
R-free | 0.16900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3l4z |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.090 | 1.830 |
High resolution limit [Å] | 1.740 | 1.740 |
Number of reflections | 109959 | |
<I/σ(I)> | 12.9 | 2.4 |
Completeness [%] | 94.7 | 96 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 291 | 32% Morpheus ethylene glycol/PEG 8000 mix, 0.05 M Morpheus carbxylic acid mix, 0.1 M Morpheus buffer system 1 |