5F07

Crystal structure of glutathione transferase F8 from Populus trichocarpa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979769
Spacegroup name	C 1 2 1
Unit cell lengths	90.010, 55.290, 55.029
Unit cell angles	90.00, 119.89, 90.00

Refinement procedure

Resolution	39.017 - 1.500
R-factor	0.1654
Rwork	0.164
R-free	0.18810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ri6
RMSD bond length	0.008
RMSD bond angle	1.182
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.710	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.045	0.292
Number of reflections	37437
<I/σ(I)>	14.9	3.5
Completeness [%]	99.6	98.4
Redundancy	3.6	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	277	25% PEG 4000, Na MES pH6.5, 0.2 M Mg chloride