5EZM

Crystal Structure of ArnT from Cupriavidus metallidurans in the apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	58.604, 80.630, 150.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.405 - 2.700
R-factor	0.2151
Rwork	0.212
R-free	0.25970
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.552
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX (dev_2236)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.410	47.410	2.830
High resolution limit [Å]	2.700	8.950	2.700
Rmerge	0.390	0.108	3.849
Rpim	0.077	0.027	0.828
Total number of observations	555326	13551	57706
Number of reflections	20291
<I/σ(I)>	63.6	503.9	1.4
Completeness [%]	100.0	99.6	99.9
Redundancy	27.4	21.2	21.9
CC(1/2)	0.995	0.997	0.221

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	295	Protein buffer: HEPES, NaCl, DDM, Tcep; Precipitant: MES, PEG500 DME, Manganese chloride, Sodium formate, Tcep; LCP lipids: 99.2% monoolein, 0.8% decaprenyl phosphate