5EZK
RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-26 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 63 |
Unit cell lengths | 255.544, 255.544, 189.392 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 143.890 - 8.500 |
R-factor | 0.47 |
Rwork | 0.470 |
R-free | 0.47000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4IGC |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER (2.5.4) |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 143.890 | 143.890 | 9.510 |
High resolution limit [Å] | 8.500 | 16.760 | 8.500 |
Rmerge | 0.200 | 0.063 | 0.640 |
Rpim | 0.089 | 0.021 | 0.668 |
Total number of observations | 91452 | 8621 | 23709 |
Number of reflections | 9102 | ||
<I/σ(I)> | 8.4 | 28.3 | 3.8 |
Completeness [%] | 99.9 | 99.7 | 99.9 |
Redundancy | 10 | 10.1 | 9.4 |
CC(1/2) | 0.996 | 0.997 | 0.600 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 8% PGA-LM, 300mM Ammonium Formate, 100mM Hepes |