5EZK

RNA polymerase model placed by Molecular replacement into X-ray diffraction map of DNA-bound RNA Polymerase-Sigma 54 holoenzyme complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-05-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 63
Unit cell lengths	255.544, 255.544, 189.392
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	143.890 - 8.500
R-factor	0.47
Rwork	0.470
R-free	0.47000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4IGC
Data reduction software	XDS
Data scaling software	Aimless (0.1.29)
Phasing software	PHASER (2.5.4)
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	143.890	143.890	9.510
High resolution limit [Å]	8.500	16.760	8.500
Rmerge	0.200	0.063	0.640
Rpim	0.089	0.021	0.668
Total number of observations	91452	8621	23709
Number of reflections	9102
<I/σ(I)>	8.4	28.3	3.8
Completeness [%]	99.9	99.7	99.9
Redundancy	10	10.1	9.4
CC(1/2)	0.996	0.997	0.600

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	8% PGA-LM, 300mM Ammonium Formate, 100mM Hepes