5EZJ

Crystal Structure of Fab of parasite invasion inhibitory antibody c1 - orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.999930
Spacegroup name	P 21 21 21
Unit cell lengths	64.810, 72.770, 109.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.915 - 1.950
R-factor	0.2006
Rwork	0.198
R-free	0.24680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ezi
RMSD bond length	0.007
RMSD bond angle	0.901
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.900		1.940
High resolution limit [Å]	1.890	8.450	1.890
Rmerge	0.120	0.043	1.920
Rmeas	0.131	0.048	2.101
Total number of observations	262881
Number of reflections	41941	559	2704
<I/σ(I)>	8.71	25.14	1.05
Completeness [%]	98.8	98.6	86.9
Redundancy	6.3		6.7
CC(1/2)	0.996	0.995	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	0.1 M HEPES/NaOH pH 7.0, 20% PEG 8000