5EZE
A de novo designed heptameric coiled coil CC-Hept-I18betaMeCys-L22H-I25E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-11-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 62.450, 60.490, 68.812 |
| Unit cell angles | 90.00, 112.43, 90.00 |
Refinement procedure
| Resolution | 43.830 - 1.800 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pna |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.861 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.833 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.071 |
| Number of reflections | 21962 | |
| <I/σ(I)> | 7.1 | 2.6 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M sodium formate with 20% w/v PEG 3350. |
