5EZ1
Crystal Structure of Cell Binding Factor 2 from Helicobacter pylori in complex with I2CA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-28 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 61.998, 61.998, 366.427 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.673 - 2.400 |
R-factor | 0.247 |
Rwork | 0.244 |
R-free | 0.29950 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.245 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 33151 |
<I/σ(I)> | 30.2 |
Completeness [%] | 98.9 |
Redundancy | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 44% Jaffamine M-600 pH 7.0, 50 mM Succinate acid pH 3.5 |