5EYW
Crystal structure of Litopenaeus vannamei triosephosphate isomerase complexed with 2-Phosphoglycolic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-05 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 |
Unit cell lengths | 38.140, 45.980, 71.850 |
Unit cell angles | 74.56, 80.24, 75.17 |
Refinement procedure
Resolution | 27.150 - 1.700 |
R-factor | 0.1942 |
Rwork | 0.192 |
R-free | 0.23660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2i9e |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.150 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.085 | 0.314 |
Number of reflections | 48059 | |
<I/σ(I)> | 15.67 | 5.46 |
Completeness [%] | 96.6 | 93.5 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | Crystallization experiments was performed with the addition of 15 mM PGA to the concentrated LvTIM protein. Needle-like crystals appeared in a reservoir solution containing 100mM Tris HCl pH 8.5, 30% w/v Polyethylene glycol 4,000 and 200mM Lithium sulfate monohydrate. Protein crystals grew for one week reaching dimensions of 400 x 50 x 50 um. |