5EYF
Crystal Structure of Solute-binding Protein from Enterococcus faecium with Bound Glutamate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.405, 115.434, 53.825 |
Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
Resolution | 31.569 - 1.520 |
R-factor | 0.148 |
Rwork | 0.146 |
R-free | 0.18100 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.056 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.520 | 1.520 |
Number of reflections | 66736 | |
<I/σ(I)> | 23.6 | 2.5 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 30% PEG 4000; 0.1M Tris-HCl pH 8.5; 0.2 M Magnesium Chloride |