5EYF
Crystal Structure of Solute-binding Protein from Enterococcus faecium with Bound Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.405, 115.434, 53.825 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 31.569 - 1.520 |
| R-factor | 0.148 |
| Rwork | 0.146 |
| R-free | 0.18100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.056 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Number of reflections | 66736 | |
| <I/σ(I)> | 23.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 30% PEG 4000; 0.1M Tris-HCl pH 8.5; 0.2 M Magnesium Chloride |
