5EY7
Crystal structure of Fructokinase from Vibrio cholerae O395 in apo form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-11-18 |
Detector | MARRESEARCH |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 107.116, 99.858, 61.548 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.409 - 2.461 |
R-factor | 0.2169 |
Rwork | 0.215 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tz6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.737 |
Data reduction software | AUTOMAR |
Data scaling software | AUTOMAR |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.450 | 2.460 |
Rmerge | 0.056 | 0.200 |
Number of reflections | 23435 | |
<I/σ(I)> | 6.7 | 1.9 |
Completeness [%] | 94.3 | 95.01 |
Redundancy | 2.73 | 2.76 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5% PEG 6000, 0.1M MES, pH 6.0 |