5EY2
Crystal structure of CodY from Bacillus cereus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97919 |
Spacegroup name | P 61 |
Unit cell lengths | 132.066, 132.066, 223.082 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.177 - 3.000 |
R-factor | 0.2393 |
Rwork | 0.238 |
R-free | 0.27740 |
RMSD bond length | 0.004 |
RMSD bond angle | 1.005 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.080 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.109 | 0.475 |
Number of reflections | 43902 | |
<I/σ(I)> | 16.83 | 3.08 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 12.1 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 100 mM Tris, 200 mM MgCl2, 20% PEG400 |