5EXS

AAA+ ATPase FleQ from Pseudomonas aeruginosa bound to ATP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	105.151, 105.151, 42.841
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	52.576 - 2.500
R-factor	0.1801
Rwork	0.178
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5exp
RMSD bond length	0.009
RMSD bond angle	1.195
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.600	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.166	0.554
Number of reflections	9612
<I/σ(I)>	6.1	1.8
Completeness [%]	99.9	99.5
Redundancy	7.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Bis-Tris pH 6.5, 0.2 M ammonium sulfate and 25% w/v PEG3350