5EXG
Crystal structure of recombinant dimeric Banana lectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 47.530, 63.330, 95.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.320 - 1.700 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.19800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bmy |
RMSD bond length | 0.019 |
RMSD bond angle | 1.882 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.5) |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.740 | |
High resolution limit [Å] | 1.700 | 7.600 | 1.700 |
Rmerge | 0.131 | 0.036 | 1.232 |
Rmeas | 0.137 | 0.038 | 1.283 |
Total number of observations | 404818 | ||
Number of reflections | 32288 | 411 | 2284 |
<I/σ(I)> | 15.77 | 51.89 | 2.07 |
Completeness [%] | 98.8 | 92.2 | 96.5 |
Redundancy | 12.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | A protein solution [10 mg/mL, in 100 mM NaCl, 20mM Tris (pH 7.4)] was mixed 1:1 (v/v) with a reservoir solution containing 0.05 M NaBr, 0.1 M Bis Tris and 19% PEG 3350 (pH 7.5) |