5EXB
Wild type green fluorescent protein DendFP (Dendronephthya sp.)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 69.384, 106.397, 137.209 |
Unit cell angles | 109.68, 100.01, 101.72 |
Refinement procedure
Resolution | 37.370 - 1.810 |
R-factor | 0.1828 |
Rwork | 0.182 |
R-free | 0.24930 |
RMSD bond length | 0.021 |
RMSD bond angle | 2.224 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.840 |
High resolution limit [Å] | 1.810 | 4.910 | 1.810 |
Rmerge | 0.033 | 0.016 | 0.244 |
Rmeas | 0.046 | 0.023 | 0.345 |
Rpim | 0.033 | 0.016 | 0.244 |
Total number of observations | 615189 | ||
Number of reflections | 310150 | ||
<I/σ(I)> | 13.5 | ||
Completeness [%] | 97.0 | 98.8 | 90.6 |
Redundancy | 2 | 2 | 1.9 |
CC(1/2) | 0.998 | 0.809 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Mg formate, 20% PEG 3350 |