5EX9
Structure of P450 StaF from glycopeptide antibiotic A47934 biosynthesis; glycerol cryo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.700, 109.700, 93.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.510 - 2.200 |
| R-factor | 0.1922 |
| Rwork | 0.191 |
| R-free | 0.22011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o1a |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.071 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 33006 | |
| <I/σ(I)> | 16.6 | 5.5 |
| Completeness [%] | 98.4 | 95.5 |
| Redundancy | 9.6 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 277 | 1.2 M NH4PO4, 0.3 M K2HPO4, 0.1 M NH4PO4 citrate (pH 4.2) |
