5EX7
Crystal structure of Brat NHL domain in complex with an 8-nt hunchback mRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9788 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 150.518, 150.518, 37.921 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.865 - 2.600 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7f |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.082 | 0.049 | 0.652 |
| Rmeas | 0.086 | 0.052 | 0.685 |
| Rpim | 0.026 | 0.016 | 0.208 |
| Total number of observations | 169589 | ||
| Number of reflections | 15443 | ||
| <I/σ(I)> | 11.6 | ||
| Completeness [%] | 99.9 | 99.1 | 100 |
| Redundancy | 11 | 10.1 | 10.7 |
| CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 289 | 14% Jeffamine ED-2001, 0.1 M imidozale |
