5EWT

Crystal structure of ExoIII endonuclease from Sulfolobus islandicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-10
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.979
Spacegroup name	P 41
Unit cell lengths	79.138, 79.138, 54.971
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.569 - 1.800
R-factor	0.1696
Rwork	0.168
R-free	0.19880
RMSD bond length	0.004
RMSD bond angle	0.753
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10pre_2124: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.700
Number of reflections	37549
<I/σ(I)>	24.2
Completeness [%]	99.9
Redundancy	7.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M bicine pH 9.0, 20% PEG 6000