5EW5
Crystal Structure of Colicin E9 In Complex with Its Immunity Protein Im9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 94.894, 116.050, 150.365 |
Unit cell angles | 90.00, 93.72, 90.00 |
Refinement procedure
Resolution | 29.600 - 3.200 |
R-factor | 0.21544 |
Rwork | 0.212 |
R-free | 0.27062 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1JCH and 2GZG |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.600 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 53941 | |
<I/σ(I)> | 7.7 | 1.8 |
Completeness [%] | 99.1 | 95.7 |
Redundancy | 3.5 | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1.3M sodium malonate, PEG3350 |