5EVY
Salicylate hydroxylase substrate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-25 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 64 |
| Unit cell lengths | 142.330, 142.330, 62.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.320 - 2.470 |
| R-factor | 0.20385 |
| Rwork | 0.201 |
| R-free | 0.26017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.757 |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.830 | 2.600 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.105 | |
| Number of reflections | 26083 | |
| <I/σ(I)> | 12 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.6 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | ammonium sulfate, lithium sulfate, Tris-HCl |
