5EVO
Structure of Dehydroascrobate Reductase from Pennisetum Americanum in complex with two non-native ligands, Acetate in the G-site and Glycerol in the H-site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-08-18 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 69.494, 69.494, 111.557 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.140 - 2.510 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.24080 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.120 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 9893 |
<I/σ(I)> | 28.99 |
Completeness [%] | 99.4 |
Redundancy | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | SODIUM ACETATE, AMMONIUM SULFATE |