5EVJ
X-ray crystal structure of CrArsM, an arsenic (III) S-adenosylmethionine methyltransferase from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-06-01 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.885, 157.885, 95.452 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.580 - 2.400 |
| R-factor | 0.18293 |
| Rwork | 0.180 |
| R-free | 0.23048 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fs8 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.032 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 34690 | |
| <I/σ(I)> | 34.1 | 6.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10% PEG 8000, 2.0 M NaCl |
