5EVG
Crystal structure of a Francisella virulence factor FvfA in the orthorhombic form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9753 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 28.702, 51.772, 74.754 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.377 - 1.820 |
| R-factor | 0.2107 |
| Rwork | 0.209 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5evf |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.714 |
| Data reduction software | XDS (January 10, 2014) |
| Data scaling software | Aimless (1.6.18) |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX ((dev_2299: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.400 | 1.860 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.064 | 0.937 |
| Number of reflections | 10549 | |
| <I/σ(I)> | 18.4 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | Citric acid, PEG 3350 |
