5EVG

Crystal structure of a Francisella virulence factor FvfA in the orthorhombic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9753
Spacegroup name	P 21 21 21
Unit cell lengths	28.702, 51.772, 74.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.377 - 1.820
R-factor	0.2107
Rwork	0.209
R-free	0.25310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5evf
RMSD bond length	0.006
RMSD bond angle	0.714
Data reduction software	XDS (January 10, 2014)
Data scaling software	Aimless (1.6.18)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX ((dev_2299: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.400	1.860
High resolution limit [Å]	1.820	1.820
Rmerge	0.064	0.937
Number of reflections	10549
<I/σ(I)>	18.4	2.2
Completeness [%]	100.0	100
Redundancy	7	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	Citric acid, PEG 3350