5EVF
Crystal structure of a Francisella virulence factor FvfA in the hexagonal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 |
| Unit cell lengths | 60.430, 60.430, 58.571 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.025 - 1.762 |
| R-factor | 0.1687 |
| Rwork | 0.167 |
| R-free | 0.19710 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.871 |
| Data reduction software | MOSFLM (7.0.9) |
| Data scaling software | Aimless (0.1.27) |
| Phasing software | SOLVE (2.13) |
| Refinement software | PHENIX ((dev_2299: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.025 | 1.800 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.039 | 0.128 |
| Number of reflections | 12091 | |
| <I/σ(I)> | 46 | 14.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.9 | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Potassium acetate, Bis-Tris Propane, PEG 1000 |
