5EUZ
Rat prestin STAS domain in complex with iodide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 2.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 61.911, 61.911, 66.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.737 - 2.403 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3llo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.793 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.740 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.161 | 1.733 |
| Number of reflections | 6054 | |
| <I/σ(I)> | 14.9 | 1.7 |
| Completeness [%] | 99.8 | 98.1 |
| Redundancy | 14.7 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M Mes pH 6.5, 1.8 M Ammonium Sulphate, 5% (w/v) PEG400, 0.1% (w/v) octyl-beta-D-glucopyranoside. Crystals were soaked with precipitant solution supplemented with 0.5 M Potassium Iodide |
