5EUP

Structure of the Drosophila melanogaster CP190 BTB domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.98038
Spacegroup name	P 32 2 1
Unit cell lengths	86.224, 86.224, 40.201
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.223 - 2.500
R-factor	0.2233
Rwork	0.219
R-free	0.25930
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.550
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.8_1069)
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.223	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	6124
<I/σ(I)>	23.3	3.3
Completeness [%]	99.5	100
Redundancy	5.3	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Well condition = 1 ml of 20% PEG 3350 (w/v), 160 mM ammonium citrate dibasic. The initial hanging drop contained 2 micro liters of the mother liquor plus 2 micro liters of 15 mg/ml CP190 BTB domain.