5EUM

1.8 Angstrom Crystal Structure of ATP-binding Component of Fused Lipid Transporter Subunits of ABC superfamily from Haemophilus influenzae.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98756
Spacegroup name	P 41
Unit cell lengths	95.237, 95.237, 60.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 1.800
R-factor	0.1727
Rwork	0.171
R-free	0.20394
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ghi
RMSD bond length	0.009
RMSD bond angle	1.405
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.048	0.640
Number of reflections	50720
<I/σ(I)>	32.8	3.4
Completeness [%]	99.8	100
Redundancy	7.5	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	Protein: 8.5 mg/ml, 0.01M Tris-HCL (pH 8.3); Screen: JCSG+ (E2), 0.2M Sodium chloride, 0.1M Sodium cacodylate (pH 6.5), 2M Ammonium sulfate; Cryo: 1:1 (screen solution) : (50% Sucrose).