5EUM
1.8 Angstrom Crystal Structure of ATP-binding Component of Fused Lipid Transporter Subunits of ABC superfamily from Haemophilus influenzae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98756 |
Spacegroup name | P 41 |
Unit cell lengths | 95.237, 95.237, 60.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.460 - 1.800 |
R-factor | 0.1727 |
Rwork | 0.171 |
R-free | 0.20394 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ghi |
RMSD bond length | 0.009 |
RMSD bond angle | 1.405 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.640 |
Number of reflections | 50720 | |
<I/σ(I)> | 32.8 | 3.4 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.5 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | Protein: 8.5 mg/ml, 0.01M Tris-HCL (pH 8.3); Screen: JCSG+ (E2), 0.2M Sodium chloride, 0.1M Sodium cacodylate (pH 6.5), 2M Ammonium sulfate; Cryo: 1:1 (screen solution) : (50% Sucrose). |