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5EUI

Structure of predicted ancestral pika hemoglobin

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 14-BM-C
Synchrotron siteAPS
Beamline14-BM-C
Temperature [K]90
Detector technologyCCD
Collection date2013-10-21
DetectorADSC QUANTUM 315
Wavelength(s)0.9787
Spacegroup nameC 2 2 21
Unit cell lengths63.350, 91.412, 103.537
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.289 - 1.450
R-factor0.195
Rwork0.194
R-free0.21140
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)2dn1
RMSD bond length0.007
RMSD bond angle1.142
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.9_1692)
Data quality characteristics
 Overall
Low resolution limit [Å]30.290
High resolution limit [Å]1.450
Rmerge0.059
Number of reflections52380
<I/σ(I)>26.96
Completeness [%]97.9
Redundancy7
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1MICROBATCH8299PEG 3350, sodium phosphate, potassium phosphate, calcium chloride

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