5EUI
Structure of predicted ancestral pika hemoglobin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2013-10-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9787 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.350, 91.412, 103.537 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.289 - 1.450 |
R-factor | 0.195 |
Rwork | 0.194 |
R-free | 0.21140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2dn1 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.142 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.290 |
High resolution limit [Å] | 1.450 |
Rmerge | 0.059 |
Number of reflections | 52380 |
<I/σ(I)> | 26.96 |
Completeness [%] | 97.9 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8 | 299 | PEG 3350, sodium phosphate, potassium phosphate, calcium chloride |