5EUF
The crystal structure of a protease from Helicobacter pylori
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97918, 0.97940 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.159, 50.266, 147.889 |
| Unit cell angles | 90.00, 94.86, 90.00 |
Refinement procedure
| Resolution | 47.803 - 2.800 |
| R-factor | 0.1894 |
| Rwork | 0.186 |
| R-free | 0.24450 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.598 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.124 | 0.701 |
| Number of reflections | 27514 | |
| <I/σ(I)> | 9 | 1.6 |
| Completeness [%] | 95.3 | 92.1 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Calcium Acetate, 0.1M HEPES:NaOH, 10% (w/v) PEG8000 |
