5ETI

Structure of dead kinase MAPK14

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-18
Detector	MAR CCD 165 mm
Wavelength(s)	1.063
Spacegroup name	P 21 21 21
Unit cell lengths	45.418, 87.023, 122.921
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.867 - 2.800
R-factor	0.1778
Rwork	0.175
R-free	0.22530
RMSD bond length	0.009
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	19.987
High resolution limit [Å]	2.800
Number of reflections	10748
<I/σ(I)>	13.3
Completeness [%]	87.9
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3.5 M Na Formate pH 7.0