5ETF
Structure of dead kinase MAPK14 with bound the KIM domain of MKK6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.383, 82.383, 123.335 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.655 - 2.400 |
| R-factor | 0.1662 |
| Rwork | 0.163 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.929 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.690 | |
| Number of reflections | 32069 | |
| <I/σ(I)> | 13.3 | 2.1 |
| Completeness [%] | 90.3 | 59 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% (w/v) PEG 3350 and 0.1 mM Bis-Tris pH6.5 |
