5ETE
Structure of pathogen-related yeast protein, Pry1 in complex with a competitive inhibitor of cholesterol binding
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-04-14 |
Detector | RIGAKU RAXIS HTC |
Wavelength(s) | 1.54056 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 124.740, 124.740, 59.060 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.485 - 2.100 |
R-factor | 0.1682 |
Rwork | 0.167 |
R-free | 0.19040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1smb |
RMSD bond length | 0.011 |
RMSD bond angle | 0.935 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2405)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.750 |
High resolution limit [Å] | 2.100 |
Number of reflections | 17583 |
<I/σ(I)> | 15.4 |
Completeness [%] | 99.8 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 1.6M Ammonium sulphate, 0.1M MES pH 6.5, 10% v/v 1,4-dioxane |