5ET9
Racemic crystal structures of Pribnow box consensus promoter sequence (P21/n)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-11 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 1 21/n 1 |
Unit cell lengths | 28.786, 68.288, 34.213 |
Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
Resolution | 34.140 - 1.830 |
R-factor | 0.2993 |
Rwork | 0.298 |
R-free | 0.32940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1FQ2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.140 | 1.895 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.016 | 0.390 |
Number of reflections | 10610 | |
<I/σ(I)> | 17.5 | 1.87 |
Completeness [%] | 92.2 | 65.75 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | DNA containing D- and L- sugars (racemic mixture), sodium cacodylate, barium chloride, potassium chloride, spermine tetrachloride, MPD |