5ET1
Crystal structure of Myo3b-ARB1 in complex with Espin1-AR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.740, 71.142, 76.883 |
| Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
| Resolution | 32.242 - 1.650 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.19110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n0r |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.795 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.916 | |
| Number of reflections | 93433 | |
| <I/σ(I)> | 18.5 | 1.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 289 | 0.2 M Lithium Acetate, 20%(w/v) PEG3350 |
