5ET0
Crystal structure of Myo3b-ARB2 in complex with Espin1-AR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 39.740, 68.780, 173.450 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 34.690 - 2.300 |
| R-factor | 0.2247 |
| Rwork | 0.223 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5et1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.111 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 173.450 | 34.690 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.041 | 0.799 | |
| Rmeas | 0.120 | ||
| Rpim | 0.062 | 0.025 | 0.469 |
| Total number of observations | 148562 | 4445 | 22264 |
| Number of reflections | 39636 | ||
| <I/σ(I)> | 7.7 | 16.6 | 1.9 |
| Completeness [%] | 94.9 | 90.5 | 96.6 |
| Redundancy | 3.7 | 3.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 1.6 M Ammonium Sulfate, 0.1 M Tris |
