5ESA
Crystal structure of anti-HCV E2 antibody HC84-26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.736, 69.806, 87.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.170 - 2.600 |
R-factor | 0.1879 |
Rwork | 0.184 |
R-free | 0.25080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.323 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.170 | 2.693 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.109 | 0.457 |
Number of reflections | 12733 | |
<I/σ(I)> | 19.8 | 6.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% w/v PEG1000, 0.1 M sodium cacodylate/HCl, pH 6.5, 0.2 M magnesium chloride |