5ES9
Crystal structure of the LgrA initiation module in the formylation state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2015-01-21 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 162.057, 162.057, 206.493 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.450 - 3.770 |
| R-factor | 0.3095 |
| Rwork | 0.308 |
| R-free | 0.33100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5es6 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.913 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.450 | 50.000 | 3.970 |
| High resolution limit [Å] | 3.770 | 10.550 | 3.900 |
| Rmerge | 0.076 | 0.038 | |
| Total number of observations | 327215 | ||
| Number of reflections | 32124 | ||
| <I/σ(I)> | 7.3 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 11.2 | 10 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1 M AmSO4, 0.1 M bis-Tris pH 5.5, 3% PEG 3350 |
