5ERC
X-ray crystal structure of BRPF1 PZP domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-27 |
Detector | NOIR-1 |
Wavelength(s) | 1.28 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 73.870, 73.870, 147.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 63.973 - 2.050 |
R-factor | 0.1651 |
Rwork | 0.162 |
R-free | 0.19340 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 63.973 |
High resolution limit [Å] | 2.050 |
Number of reflections | 15595 |
<I/σ(I)> | 19.6 |
Completeness [%] | 100.0 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Calcium acetate, 0.1 M Imidazole pH 8.0, 10% PEG8000 |