5ERB
Ketosynthase from module 5 of the bacillaene synthase from Bacillus amyloliquefaciens FZB42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-02 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.758, 319.132, 103.470 |
Unit cell angles | 90.00, 110.26, 90.00 |
Refinement procedure
Resolution | 97.070 - 4.200 |
R-factor | 0.29112 |
Rwork | 0.288 |
R-free | 0.34088 |
RMSD bond length | 0.013 |
RMSD bond angle | 2.651 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 97.070 |
High resolution limit [Å] | 4.200 |
Number of reflections | 23712 |
<I/σ(I)> | 14.2 |
Completeness [%] | 79.9 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | 1.75 uL protein solution (9.9 mg/mL BaeKS5, 150 mM NaCl, 15 mM HEPES, pH 7.5) with 0.5 uL crystallization buffer (1.1 M LiSO4, 0.1 M Tris, pH 8.0) |