5EQZ

Crystal structure of a Rev protein from Borrelia burgdorferi at 1.80 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-25
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	63.970, 42.510, 64.140
Unit cell angles	90.00, 109.21, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.2071
Rwork	0.203
R-free	0.24210
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.716
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.030	0.018	0.519
Rmeas	0.032	0.019	0.578
Total number of observations	101069
Number of reflections	15025	179	1053
<I/σ(I)>	34.87	98.46	3.49
Completeness [%]	98.2	96.8	92.5
Redundancy	6.7		4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	290	native crystal, MCSG1 condition F03: 20% PEG 3350,0.2 M Ammonium Citrate Dibasic, pH 5.0, BobuA.18943.a.B2.PS02467 at 19.33 mg/ml, cryo 20% EG, tray 266003F3, puck pla5-11. Iodide data set,JCSG+ condition A03: 20% Peg 3350, 200 mM Ammonium Citrate Tribasic, BobuA.18943.a.B2.PS02467 at 19.33 mg/ml, cryo 30% EG, 1.5 M NaI, tray 266002A3, puck pla5-6
1	VAPOR DIFFUSION, SITTING DROP	5	290	native crystal, MCSG1 condition F03: 20% PEG 3350,0.2 M Ammonium Citrate Dibasic, pH 5.0, BobuA.18943.a.B2.PS02467 at 19.33 mg/ml, cryo 20% EG, tray 266003F3, puck pla5-11. Iodide data set,JCSG+ condition A03: 20% Peg 3350, 200 mM Ammonium Citrate Tribasic, BobuA.18943.a.B2.PS02467 at 19.33 mg/ml, cryo 30% EG, 1.5 M NaI, tray 266002A3, puck pla5-6