5EQL
Isoform-specific inhibition of SUMO-dependent protein-protein interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 74.270, 74.270, 59.717 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 64.320 - 2.490 |
| R-factor | 0.21965 |
| Rwork | 0.218 |
| R-free | 0.24265 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1WM3 and 5ELJ |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.531 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.320 | 2.610 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.062 | 0.454 |
| Number of reflections | 6930 | |
| <I/σ(I)> | 17.7 | 3.8 |
| Completeness [%] | 99.5 | 96.7 |
| Redundancy | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 291 | 0.1 M HEPES sodium salt pH 7.6, 22% w/v polyethylene glycol 3350 |
