5EQ9
Crystal structure of Medicago truncatula Histidinol-Phosphate Phosphatase (MtHPP) in complex with L-histidinol phosphate and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.885, 89.802, 92.593 |
Unit cell angles | 90.00, 97.07, 90.00 |
Refinement procedure
Resolution | 36.250 - 1.360 |
R-factor | 0.116 |
Rwork | 0.116 |
R-free | 0.15600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.020 |
RMSD bond angle | 1.900 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.440 |
High resolution limit [Å] | 1.360 | 1.360 |
Rmerge | 0.072 | 0.820 |
Number of reflections | 214644 | |
<I/σ(I)> | 15 | 1.9 |
Completeness [%] | 99.8 | 98.8 |
Redundancy | 5.1 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | Protein at 19 mg/ml concentration. 15% PEG 3350, 0.2 M diammonium hydrogen phosphate, pH 8.0. Crystal washed in a crystallization solution supplemented with 2 mM MgCl2, 5 mM HOLP and 20% glycerol and immediately vitrified in liquid nitrogen |