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5EQ9

Crystal structure of Medicago truncatula Histidinol-Phosphate Phosphatase (MtHPP) in complex with L-histidinol phosphate and Mg2+

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 19-ID
Synchrotron siteAPS
Beamline19-ID
Temperature [K]100
Detector technologyCCD
Collection date2015-07-30
DetectorADSC QUANTUM 315r
Wavelength(s)0.9793
Spacegroup nameP 1 21 1
Unit cell lengths61.885, 89.802, 92.593
Unit cell angles90.00, 97.07, 90.00
Refinement procedure
Resolution36.250 - 1.360
R-factor0.116
Rwork0.116
R-free0.15600
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.020
RMSD bond angle1.900
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareREFMAC (5.8.0131)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.0001.440
High resolution limit [Å]1.3601.360
Rmerge0.0720.820
Number of reflections214644
<I/σ(I)>151.9
Completeness [%]99.898.8
Redundancy5.15
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8292Protein at 19 mg/ml concentration. 15% PEG 3350, 0.2 M diammonium hydrogen phosphate, pH 8.0. Crystal washed in a crystallization solution supplemented with 2 mM MgCl2, 5 mM HOLP and 20% glycerol and immediately vitrified in liquid nitrogen

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