5EQ1
Crystal structure of the human BRPF1 bromodomain in complex with SEED12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.380, 60.380, 63.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.288 - 1.550 |
| R-factor | 0.1888 |
| Rwork | 0.189 |
| R-free | 0.19370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.041 | 0.338 |
| Number of reflections | 19761 | |
| <I/σ(I)> | 25.9 | 6.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium nitrate, 20% PEG3350 |
