5EPZ
Human Angiogenin in complex with sulphate anions at a basic solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0403 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 82.330, 37.420, 42.580 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.580 - 1.850 |
| R-factor | 0.18483 |
| Rwork | 0.183 |
| R-free | 0.21749 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eop |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.369 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.580 | 1.890 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.518 |
| Number of reflections | 11802 | |
| <I/σ(I)> | 22.4 | 6.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 289 | 0.1 M Tris/HCl buffer pH 8.0, 2.4 M ammonium sulphate |
