5EPW
C-Terminal Domain Of Human Coronavirus Nl63 Nucleocapsid Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I23 |
| Synchrotron site | Diamond |
| Beamline | I23 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918409 |
| Spacegroup name | P 61 |
| Unit cell lengths | 68.290, 68.290, 92.494 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.830 - 1.500 |
| R-factor | 0.17008 |
| Rwork | 0.169 |
| R-free | 0.19768 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.395 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.830 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.085 | 0.841 |
| Number of reflections | 39167 | |
| <I/σ(I)> | 15.3 | 3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M PCTP pH 8.0, 25% PEG 1500 |
