5EPV
Histidine kinase domain from the LOV-HK blue-light receptor from Brucella abortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98011,1.80000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.390, 100.840, 71.410 |
Unit cell angles | 90.00, 102.75, 90.00 |
Refinement procedure
Resolution | 48.550 - 2.510 |
R-factor | 0.2039 |
Rwork | 0.202 |
R-free | 0.24220 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.510 | 2.510 |
Rmerge | 0.060 | 0.591 |
Number of reflections | 32760 | |
<I/σ(I)> | 14.3 | 2 |
Completeness [%] | 97.7 | 95.1 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 295 | 7.5% w/v PEG8000, 0.1 M HEPES, pH 8.3 |