5EPP

Structural Insights into the Interaction of p97 N-terminus Domain and VBM Motif in Rhomboid Protease, RHBDL4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 65
Unit cell lengths	96.999, 96.999, 50.902
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.002 - 1.880
R-factor	0.1784
Rwork	0.177
R-free	0.20950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qq7
RMSD bond length	0.007
RMSD bond angle	0.989
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.880
Number of reflections	22279
<I/σ(I)>	8.2
Completeness [%]	99.4
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG 3350, 0.1 M Bis-Tris Propane pH 6.5, 0.2 M Sodium Acetate