5EPO

The three-dimensional structure of Clostridium absonum 7alpha-hydroxysteroid dehydrogenase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-12-04
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5419
Spacegroup name	P 21 21 21
Unit cell lengths	67.180, 69.680, 220.390
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.580 - 2.000
R-factor	0.18411
Rwork	0.182
R-free	0.22189
RMSD bond length	0.019
RMSD bond angle	1.565
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	110.195
High resolution limit [Å]	1.726
Number of reflections	69768
<I/σ(I)>	7.1
Completeness [%]	98.3
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.0M Ammonium citrate tribasic, 0.1M BIS-TRIS propane, PH 7.0