5EPK
Crystal Structure of chromodomain of CBX2 in complex with inhibitor UNC3866
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-22 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 |
| Unit cell lengths | 78.894, 78.894, 30.609 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.000 - 1.800 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.21200 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.892 |
| Data scaling software | Aimless (0.5.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.160 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.886 |
| Number of reflections | 10262 | |
| <I/σ(I)> | 29.2 | 3.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.6 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2 M potassium thiocyanate |
